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KMID : 0379519980140040577
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한국독성학회지
1998 Volume.14 No. 4 p.577 ~ p.585
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Induction of Oxidative Stress and Cytoskeleton Damage by Cadmium in WB-F344 Rat Liver Epithelial Cells
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Jeong Sang-Hee
Cho Myung-Haeng
Cho Joon-Hyoung
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Abstract
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Cadmium is an important industrial and environmental pollutant and has adverse effects on cell growth and metabolism, although the mechanisms of its cellular toxicity are still unclear. This study was performed to elucidate the cytotoxic mechanism of cadmium in the viewpoint of oxidative stress and cytoskeleton alterations in WB-F344 rat liver epithelial cells. 200 mutextrm{M} CdCl_2 caused a severe disassembling of microtubule and micro filament and an apparent cell retraction under an observation with fluorescence micoscope. (equation omitted)-tubulin and F-actin protein were highly thiolated at 20 min and then disappeared from 1 hour after the treatment of 200 muM CdCl_2in the immunoblot analysis. Intracellular GSH was decreased from 1hr to 24 hrs by 66.6 or 200 mutextrm{M} of CdCl_2. Intracellular protein thiol was also decreased by 22.2, 66.6 and 200 mutextrm{M} of CdCl_2 at 1 hour after its treatment. The product of lipid peroxidation (malondialdehyde) was increased from 4 hrs by 66.6 and 200mutextrm{M} of CdCl_2. These data indicate that cadmium induces oxidative stress involving disassembling of microtubule and micro filament, thiolation of (equation omitted)-tubulin and actin protein, depletion of GSH and protein thiol, and increase of lipid peroxidation.
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KEYWORD
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Cadmium, Oxidative stress, GSH, Protein thiol, Malondialdehyde, Cytoskeleto
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